SARS-CoV-2 (2019-NCoV) S1 (D614G), His tagged Spike S1 Recombinant Protein

To support COVID-19 research, we produce recombinant SARS-CoV-2 proteins which can be used as antigen for analytic and immuno-diagnostic purposes and also for vaccine development.

Catalog # NTSB-1001

Why choose Neuome SARS-CoV-2 Spike Protein?

  • Val16-Lys1211 with a C-term His tag.
  • Stabilizing mutations K986P and V987P promote the prefusion conformation.
  • Two mutations, R682S and R685S, eliminate a Furin protease cleavage site.
  • Guaranteed Bioactivity and High Purity: Bioactivity tested by functional ELISA and purity determined by SDS-PAGE to be greater than 95% (range 95% – 97%).
  • Lot-to-Lot Consistency: Stringent QC testing performed on each lot to ensure consistent activity and purity.
  • Bulk Quantities Available: Bulk up and save with large mass quantities to meet your research needs. Supply agreements available, partner with us. Please contact us.
  • Our endeavor is to be the best and fastest recombinant proteins provider to the scientific community, with high quality, activity, and purity.

Product Datasheets

Product Information

Applications
Immunocytochemistry (ICC/IF)
Flow Cytometry (Flow)
ELISA (ELISA)
Product Specifications
Species Reactivity
Protein Construction
The DNA sequence encoding recombinant SARS-CoV-2 (2019 nCoV) S1 (D614G) -
Protein [His (YP_009724390.1) (Val16 Arg685(D614G))
was expressed with a polyhistidine tag at the Cterminus and was purified.
Expressed Host
HEK293 Cells
Class
C-Terminus
Type
Protein / Antigen
Clone
Immunogen
Conjugate
Form
Concentration
Purification
Storage buffer
Contains
More Details
Purity
> 98% based on SDS-PAGE determination
> 98% based on Rev-HPLC determination
Endotoxin Level
< 1.0 EU/μg protein - LAL method based detection
Activity
The binding capacity is measured by functional ELISA.
The human receptor protein ACE2 (Fc tag) at 2 μg/mLis immobilized 100μL/well that can bind 2019-nCoV S1(D614G)h,
the EC50 of 2019-nCoV S1(D614G) his 250-650 ng/mL.
Source
Novel coronavirus (2019-nCoV) or SARS CoV-2
N-terminal Sequence Analysis
Val16
Predicted Molecular Mass
76.41 kDa
SDS-PAGE
Formulation Details
Formulation
Lyophilized from Sterile Phosphate buffer saline (pH 7.4)
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products.
Please refer to it for detailed information.
Shipping
Stability & Storage
store at -20℃ to -80℃ under sterile conditions

Protein Description

The coronaviruses possess with glycoprotein protrusions called spike (S) that interact with specific receptors present on host cell. These specific receptors to which S1 binds includes ACE2, (angiotensin-converting enzyme 2), APN (aminopeptidase N), CEACAM (carcinoembryonic antigen-related cell adhesion molecule 1), DPP4 (dipeptidyl peptidase- 4), Sia (sialic acid), and O-ac Sia (O-acetylated sialic acid). The spike play significant role in viral infection by specifically establishing interaction between host and virus. The heterologous groups of viral surfsce proteins function together are termed as ‘peplomer’.The glycoprotein of coronaviruses, spike (S) is found to be crucial in initiation of infection process as it bind to the host cell receptors. The infection of human respiratory epithelial cells of by SARS-CoV-2 or COVID-19 coronavirus (2019-nCoV) is mediated through the interaction of viral spike protein with the human cell ACE2 receptor.

The spike protein made of S1 and S2 subunits is a large type I transmembrane protein. The receptor binding domain (RBD) present in S1 subunit is needed for cell surface receptor recognition. Basic elements of S2 domain are responsible for membrane fusion. The S protein plays important role in T-cell responses, neutralizing-antibody induction and also in protective immunity.

Additionally, spike protein play key role in defines viral hosts range, viral specificity, binding with the neutralizing antibody. the spike is main target for vaccine design and is transformed one host to other via mutation or gene recombination of the receptor binding domain (RBD), resulting in higher mortality rate.

Data Images

Reverse Phase HPLC

A higher resolution chromatogram of the proteoform. Our protein is offered at the highest purity range, 95% – 97%.

SDS page

SDS-PAGE and Western Blot were used for precise determination of the presence of the spike protein and as QC Check for the batches. Our protein is offered at the highest purity range, 95% – 97%.

WESTERN BLOT

SDS-PAGE and Western Blot were used for precise determination of the presence of the spike protein and as QC Check for the batches. Our protein is offered at the highest purity range, 95% – 97%.

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